Table II. LC-MS/MS data showing 14-3-3 family isoforms that copurify with variants of HopQ1-Strep II transiently expressed in N. benthamiana leaves.
14-3-3 Isoform | HopQ1-S51A (53, 43)a |
HopQ1-S49A (45, 63) |
||
---|---|---|---|---|
a | 0b | 0 | 43 | 61 |
b | 0 | 0 | 29 | 34 |
c | 0 | 0 | 50 | 47 |
d | 0 | 0 | 13 | 0 |
e | 0 | 0 | 54 | 38 |
f | 0 | 0 | 27 | 34 |
g | 0 | 0 | 23 | 14 |
h | 0 | 0 | 46 | 46 |
i | 0 | 0 | 31 | 26 |
Protein coverage parameters calculated by Mascot software are shown for each experiment. Protein coverage parameters for HopQ1-S51A and HopQ1-S49A in these experiments are shown in parentheses. bProtein coverage 0 refers to the 14-3-3 isoform that has not been identified by LC-MS/MS analyses in this experiment.