. 2013 Feb 8;161(4):2049–2061. doi: 10.1104/pp.112.209023

Table III. FRET-FLIM analysis showing that HopQ1 Ser-51 is critical for 14-3-3a binding.

Donor	Acceptor	τ^a	E^b
		ns	%
eCFP	–	2.37 ± 0.02	–
eCFP-eYFP^c	eCFP-eYFP	1.66 ± 0.09	30
14-3-3a-eCFP	–	2.36 ± 0.05	–
14-3-3a-eCFP	HopQ1wt-eYFP	2.02 ± 0.08	15
14-3-3a-eCFP	HopQ1-S51A-eYFP	2.23 ± 0.05	5

τ, Mean donor lifetimes ± sd in the presence (τ_DA) or absence (τ_D) of the acceptor. ^bE, FRET efficiency; E = 1 − (τ_DA/τ_D) × 100%. ^cThe positive control, the eCFP-eYFP fusion protein, establishes a maximal detectable level of FRET under the imaging conditions used.