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. 2013 Feb 11;161(4):2085–2101. doi: 10.1104/pp.112.212191

Figure 5.

Figure 5.

Comparison of CHRC levels in wild-type (WT) and hp1 fruits. Unless specifically indicated, equal amounts of protein were loaded on all the immunoblots. A, Relative spot volume of CHRC protein in wild-type and hp1 fruits at different stages of ripening. B, Real-time PCR quantification of CHRC transcript in wild-type and hp1 fruits at different stages of ripening. C, Immunoblotting of proteins from bell pepper and tomato fruits. The polyclonal antibody raised against bell pepper PAP recognized a protein of 35 ± 2 kD in tomato. Since PAP levels were very high in bell pepper, a reduced amount of protein (0.3 μg) was loaded compared with wild-type tomato fruits (10 μg). D, Comparison of CHRC levels in wild-type and hp1 fruits at the RR stage. Progressively increasing concentrations of proteins were loaded on the gel. The equivalence in the band intensity of hp1 (2.5 μg) with that of the wild type (5.0 μg) indicates that the CHRC level in hp1 is approximately 2-fold higher than in the wild type. E, Comparison of CHRC levels in wild-type and hp1 fruits at MG and RR stages. Ripening stimulated an increase in the level of CHRC protein from MG to RR stages, with higher CHRC levels in hp1 fruits. F, Comparison of CHRC levels in RR fruits of AC, cv Arka vikas (AV), and nor. Note that the nor mutant, although it accumulates very little lycopene, exhibits a normal level of CHRC equal to the other two cultivars. G, Relative abundance of CHRC protein in hp1 and hp2 fruits at the MG stage. H, Relative levels of CHRC in hp1, hp2, and Ip fruits at the RR stage. Note that hp1 and hp2 exhibit similar levels of CHRC, while Ip shows an intermediate level of CHRC between the wild type and hp1 and hp2 fruits. I, Analysis of CHRC levels in RR fruits of the wild type, hp3, and hp1. The order of abundance of CHRC is hp1 > hp3 > wild type. J, Comparison of CHRC transcript levels in RR fruits of hp1, hp2, hp3, and Ip in relation to the wild type. Note that all the mutants exhibit high CHRC transcript level compared with the wild type.