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. 2013 Apr 2;4:58. doi: 10.3389/fphys.2013.00058

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Copyright © 2013 Eberle, Richter, Widmayer, Chubanov, Gudermann and Breer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Acetylcholine synthesizing cells express the vesicular protein SNAP25. (A–C) Assessment of SNAP25 and ChAT-immunostaining on cross sections through the corpus mucosa at fundus/corpus boundary using specific antibodies against ChAT (red) and SNAP25 (green). ChAT-immunostaining visualized clustered ChAT-expressing cells extending into the “gastric groove” built by the “limiting ridge” (A). The SNAP25 antibody reveals a pattern of immunostaining (B) reminiscent of that of ChAT (A). Overlay (C) of (A) and (B) clearly exposes coexpression of ChAT and SNAP25 in all stained corpus mucosal cells underneath the “limiting ridge.” Sections were counterstained with DAPI (blue). Scale bars: (A–C) = 20 μm.