Figure 1.

CLF mediates the trimethylation of H3K27 at KNOX loci. ChIP experiments show that H3K27me3 levels across BP (left panel) and KNAT2 (right panel) are reduced in clf seedlings compared with wild type. Quantitative PCR values (mean ± SE) are shown as percentage of input and calculated from at least three independent biological replicates. Schematic representations of the BP and KNAT2 loci are shown at the top. (Black dashes) Positions of amplicons analyzed in ChIP assays; (red ovals) AS1–AS2 complex-binding sites; (arrow) transcription start site; (light blue boxes) 5′ and 3′ untranslated regions (UTRs); (dark blue boxes) ORF. Values on the X-axis are distances in kilobases relative to the transcription start site.