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. 2013 Mar 15;27(6):596–601. doi: 10.1101/gad.211425.112

Figure 2.

Figure 2.

The AS1–AS2 complex influences chromatin structure at BP and KNAT2. (A) Levels of H3K27me3 at BP (left panel) and KNAT2 (right panel) are significantly reduced in as1 and as2 leaves compared with wild type. (B) Occupancy levels of the PRC1 component LHP1 at BP (left panel) and KNAT2 (right panel) are likewise reduced in as2. (C) Enrichment levels for the active chromatin mark H3K4me3 across BP (left panel) and KNAT2 (right panel) in wild type, as1, and as2. Values (mean ± SE; n ≥ 3) are normalized to H3 levels (A,C) to correct for possible variation in nucleosome density at BP and KNAT2 or calculated as percentage of input (B). Values significantly different from wild type in at least one of the mutants are indicated. (*) P < 0.05; (**) P < 0.01. Schematic representations of the BP and KNAT2 loci are as in Figure 1. Control experiments establishing the specificity and efficiency of ChIP reactions are shown in Supplemental Figure 1.