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. 2013 Mar 15;27(6):627–638. doi: 10.1101/gad.212738.112

Figure 5.

Figure 5.

Prp22 and Prp43 promote spliceosomal cleavage. (A) Northern blot analysis of reporter containing wild-type TER1 intron. RNA was isolated from prp22+ and prp22T665A cells initially grown at 32°C to 5 × 106 cells per milliliter, shifted to 22°C, and harvested immediately. First-step efficiencies were calculated as cleaved plus spliced form over all forms normalized to wild type at 32°C multiplied by 100. Second-step efficiencies were calculated as spliced form over cleaved plus spliced form normalized to wild type at 32°C multiplied by 100. Data are represented as mean ± SEM (n = 3). (B) Northern blot analysis of a second Prp22 mutant prp22S663A. Analysis of TER1 processing is as in A, with n = 3. (C) Analysis for prp43S229A and prp43R406N by Northern blotting. Quantification is as described in A, with n = 2.