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. 2013 Apr 1;201(1):23–32. doi: 10.1083/jcb.201301006

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© 2013 Gascoigne and Cheeseman

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Figure 4. — CDK activity drives kinetochore assembly and disassembly. (A–E) Quantification of centromeric GFP intensity in cells exiting (A, B, D, and E) or entering (C) mitosis. Cells were imaged 48 h after depletion of PP2R2A–B55-α and immediately after treatment with 5 µM flavopiridol (CDKi; A and B), 30 min after treatment with 5 µM flavopiridol (CDKi) and immediately after treatment with 1 µM okadaic acid (C), and 30 min after treatment with 50 µM cantharidic acid or 3 µM tautomycin and immediately after treatment with 5 µM flavopiridol (CDKi; D and E). n ≥ 8 cells. Error bars represent SEM.