Figure 6.

γ-Catenin promotes TCF/LEF-mediated transcription in leukemic cells both directly and indirectly through stabilization of β-Cat. (a) Representative western blot showing the expression and localization of γ- and β-catenin protein in K562 cells transduced with empty vector (Cont), γ-Catenin or γ-catenin together with β-catenin small hairpin RNA (γ/β-Cat KO). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and histone H1 demonstrate relative protein loading and fraction purity. (b) Representative flow cytometric histograms showing TCF reporter activity in K562 cell lines. (c) Summary data showing the background-subtracted TCF reporter signal (n=3). Data represents mean±1 s.d. Statistical significance is denoted by ^†P<0.01 and ^‡P<0.05, as analyzed by Student's t-test.