Figure 3.

MYCT1 expression promotes population expansion without altering lineage distributions in bulk liquid culture. Following retroviral transduction, MYCT1 and GFP control transgenic CD34⁺ cells were cultured in medium supplemented with 5 ng/ml IL-3, SCF, G-CSF and GM-CSF for 30 days at 37 °C. At the time-points indicated, liquid cultures were analysed by four-colour immunophenotypic analysis. (a) MYCT1-expressing hematopoietic progenitor cells demonstrate increased growth over 30 days in culture compared with GFP control cells. (b) MYCT1-expressing and GFP control cells generate analogous proportions of erythrocyte (CD13^lo CD36^hi), monocyte (CD13^hi CD36^hi) and granulocyte (CD13^hi CD36^lo) populations on days 6 and 9 of bulk culture. There is no differential retention of the CD34⁺ progenitor cell marker. Data represents mean plus±1 s.d. (n=3) and statistical significance was calculated by paired t-test, ^*P<0.05 and ^†P<0.01.