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. 2013 Mar 6;136(4):1128–1138. doi: 10.1093/brain/awt037

Figure 3.

Figure 3

(A) Spreading of phosphorylated α-synuclein pathology on the contralateral side of mouse brain injected with α-synuclein fibrils. Staining of dentate gyrus and amygdala in the right hemisphere (injection side) and in the left hemisphere (non-injected side) with anti-phosphorylated α-synuclein (psyn) antibody, 1175, at 15 months after injection. (B) Distribution of phosphorylated α-synuclein pathology in human α-synuclein fibril-injected mouse brain at 15 months after injection (n = 24). Four coronal sections were stained with phosphorylated α-synuclein antibody, 1175. Red dots indicates Lewy bodies- and Lewy neurites-like pathology. Near the injection level (bregma -3.40 mm), abundant phosphorylated α-synuclein pathology was present in substantia nigra, hippocampus, external capsule, and entorhinal cortex in right hemisphere, whereas in the left hemisphere, sparser pathology was detected in hippocampus and external capsule. At the level of -1.94 mm from bregma, severe phosphorylated α-synuclein pathology was present in hippocampus, amygdala, corpus callosum, hypothalamus and motor, visual, somatosensory, auditory and piriform cortex in the right hemisphere, whereas moderate phosphorylated α-synuclein pathology was observed in corpus callosum, hippocampus, external capsule and motor, somasosensory and auditory cortex in the left hemisphere. At the level of -0.58 mm from bregma, phosphorylated α-synuclein pathology was detected in amygdala, corpus callosum, fimbria, fornix, hypothalamus, striatum and somatosensory and piriform cortex in the right hemisphere, whereas in the left hemisphere, the pathology was present in corpus callosum, fimbria, fornix, hypothalamus and striatum. At the level of 0.02 mm from bregma, phosphorylated α-synuclein pathology was concentrated in stria terminalis, septal nucleus and cingulate, motor and somatosensory cortex in the right hemisphere. In the left hemisphere, phosphorylated α-synuclein pathology was detected only in septal nucleus. Dashed box indicates substantia nigra (injection site). L = left hemisphere of brain; R = right hemisphere.