Figure 1.

Experimental design schematic. (A) At around 42 days of age, mice with differing genotypes are infected with an adenovirus expressing Cre-recombinase (Adeno-Cre) via intranasal inhalation. Eight weeks after infection mice develop tumors large enough to be visible by micro-CT imaging. Mice are imaged again at 10 weeks after Adeno-Cre infection to ensure appropriate identification of tumors with growth kinetics suggestive of lung adenocarcinoma. Immediately after a second micro-CT scan, mice undergo whole thorax irradiation with differing treatment regimens. Two weeks after radiation treatment mice are imaged again to evaluate tumor growth delay following radiation treatment. [(B) Top Panel] Image of custom built Micro-CT scanner developed at the Duke Center for In vivo Microscopy with isotropic resolution of 88 μ. [(B) Bottom Panel] Reconstructed image series were utilized to contour tumors to calculate gross tumor volumes and monitor relative growth kinetics. SC, spinal cord; H, heart.