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. 2013 Apr 2;3:72. doi: 10.3389/fonc.2013.00072

Figure 2.

Figure 2

Lung cancers from mice with conditional mutations in LSL-Kras; Ink4a/ARFFL/FL and LSL-Kras; p53FL/FL have different levels of p53 expression, but similar growth rates. Hematoxylin and eosin stained slides of the lung (200×) demonstrate histologically similar adenocarcinomas in (A) LSL-Kras; Ink4a/ARFFL/FL and (B) LSL-Kras; p53FL/FL mice. (C) Quantitative RT-PCR shows significantly different levels of p53 expression in lung cancers from LSL-Kras; Ink4a/ARFFL/FL mice (n = 11 tumors) when compared to LSL-Kras; p53FL/FL mice (n = 11 tumors, P < 0.0001). (D) Relative tumor growth over time with no treatment demonstrates similar growth rates in each tumor cohort at 56, 70, and 84 days following intranasal Adeno-Cre infection. Blue – lung cancers (n = 27) from LSL-Kras; Ink4a/ARFFL/FL mice, Red – lung cancers (n = 45) from LSL-Kras; p53FL/FL mice. (E) Serially reconstructed Micro-CT scans at 56, 70, and 84 days after Adeno-Cre infection from a LSL-Kras; p53FL/FL mouse documents clear and measurable tumor growth over time. T, tumor; H, heart. Error bars – SEM.