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(a) Schematic representation of covalent immobilization of Ply500 on silica nanoparticles followed by co-polymerization with HEMA to form an anti-listeria polymeric film. Storage stability of native Ply500 (20 μg) (♦) and Ply500-SNP (30 μg) (□) in sterile PBS at (b) 4°C and (c) 25°C. The amount (in μg) represents the Ply500 content of the formulation. Error bars represents standard deviations from triplicate measurements.
