Table 1. Anti-listeria activity of different Ply500 formulations against L. innocua assessed after incubation with 1 mL of a suspension containing 105 CFU/mla.
Ply500 Formulation | Log CFU reduction after 24 h | Retention of native enzyme activity (plating assay) as a percentage relative to native enzyme | Retention of native enzyme activity (cell wall assay) as a percentage relative to native enzyme |
---|---|---|---|
Ply500 (20 μg/ml) | 4.7 ± 0.05 | 100% | 100% |
Ply500-SNP (60 μg/ml) | 4.6 ± 0.02 | 33 | n.d.b |
Ply500 film (500 μg/ml) | 1.6 ± 0.12 | 3.9 ± 0.05 | n.d.b |
FP (20 μg/ml) | 0.3 ± 0.02 | 51 ± 4 | 4.5 ± 0.5 |
FP10 (20 μg/ml) | 2.5 ± 0.30 | 99 ± 0.5 | 32 + 4.0 |
FP10-starch nanoparticle (80 μg/ml) | 2.9 ± 0.18 | 24 ± 0.02 | n.d.b |
aListeria cells were grown in BHI media for 7 h. Standard deviations were calculated from triplicate measurements. No significant killing was observed with controls of SNP-NCO, overnight wash of Ply500-SNP, Film (without Ply500), overnight wash of Ply500 film, MBP, starch nanoparticles, and MBP-starch nanoparticles. The concentration (in μg/mL) in column one represents the Ply500 content of the formulation.
bnot determined.