Figure 2.

Apoptosis of OS cells cotreated with SCH and AAG. (a) OS1002 and OS1003 cells received 10 nℳ SCH, 5 mℳ AAG, or both for 48 h. Error bars indicate S.D. Asterisk indicates significant P-value. P-values for OS1002 cells are: SCH, 0.15; AAG, 0.59; both, 0.0019. P-values for OS1003 cells are: SCH, 0.14; AAG, 0.05; both, 0.0003. C: control. (b–e) Cells received the indicated concentrations of SCH and AAG for 48 h. Black bars denote cells receiving SCH alone. (f) OS1004 cells received the indicated concentrations of SCH and DMAG for 48 h. Black bars denote cells receiving SCH alone. (a–f) Percent apoptotic cells was determined by FACS analysis of annexin V-stained cells