Figure 4.

Survival of normal human osteoblasts and fibroblasts cotreated with SCH and AUY. (a) OS1002 and hFOB1.19 cells received 10 nℳ SCH and 25 or 100 nℳ AUY for 24 h. Percent apoptotic cells was determined by FACS analysis of annexin V-stained cells. (b, c) OS1003 and WI38 cells received 10 nℳ SCH and 100 nℳ AUY for 24 h. Percent apoptotic cells was determined by FACS analysis of annexin V-stained cells (b). Caspase-3 cleavage were determined by western blotting of cell extracts with antibody to caspase-3 (c). C: control