Figure 3.

Survival of NSCLC cells in response to TRAIL treatment. (a and b) Cells were seeded in 6-well plates, and 24 h after the seeding, cells were treated with TRAIL (200 ng/ml). At 14 days after treatment, cells were fixed in paraformaldehyde and colonies were counted. (c) A549 cells were repeatedly treated for 3 h with TRAIL (200 ng/ml). At 48 and 96 h after the initial treatment, fresh TRAIL (200 ng/ml) was added for 3 h and cells were collected. Procaspase-8 processing and PARP-1 cleavage was analyzed by immunoblot. Error bars represent S.E. *P<0.05. Cl, cleaved; Unt, untreated