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Minocycline inhibited the expression of M1 markers in the progressive disease phase. RNA was extracted from the lumbar spinal cords. The temporal mRNA expression profiles of M1 markers ((a) CD68, (b) CD86, (c) TNF-α, (d) IL-1β, and (e) IFN-γ) were examined by quantitative RT-PCR. Open columns, SOD1G93A mice (n=4); closed columns, minocycline-treated SOD1G93A mice (n=3). Error bars, S.E. **P<0.01, *P<0.05. #P<0.05, ##P<0.01 compared with 9 weeks mice
