Figure 6.

Puma is required for activated microglia/TNFα-induced NPC apoptosis. (a) NPCs derived from Puma^+/+ and Puma^−/− embryos were maintained in unconditioned stem cell media (C) or treated with increasing concentrations of LPS-activated microglia conditioned media (MCM) and the fraction of apoptotic cells was determined by Hoechst staining at 72 h (n=5, *P<0.05, **P<0.01). (b) Representative images of Hoechst staining in Puma^+/+ and Puma^−/− NPCs treated for 72 h with LPS-activated microglia conditioned media (a-MCM). Scale bar, 20 μm. (c) Puma^+/+ and Puma^−/− NPCs were treated with the indicated concentrations of rTNFα and the fraction of apoptotic cells was determined by Hoechst staining at 72 h (n=5, *P<0.05)