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. 2013 Mar 7;4(3):e533. doi: 10.1038/cddis.2013.61

Figure 8.

Figure 8

Nutlin-3 reduces TSA's ability to cause G2/M arrest and endoreduplication in tumor cells but does not protect tumor cells from TSA-mediated cytotoxicity. (a and b) HCT116 p53+/+ or HCT116 p53−/− cells were mock-treated (EtOH) or treated with Nutlin-3 (5 μM) for 24 h after which TSA (0.5 μM) was added for 30 h. Cell cycle distribution was determined by two-dimensional flow cytometry for BrdU and PI. (c) HCT116 p53+/+ cells were treated for 24 h with TSA and/or Nutlin-3 (5 μM), the latter being added 1 h after TSA treatment. Afterwards, cells were washed with growth medium and allowed to grow for 9 days. Fixed cells were stained with Giemsa solution. The histogram shows plate area occupied by cell colonies as quantified in ImageJ; plate area occupied by mock-treated cells was set at 100%. Error bars represent standard deviation of three independent experiments. *P<0.05; ****P<0.001 (unpaired two-tailed Student's t-test; n=3)