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Immunological detection of NFK in TcAChE samples light-irradiated in the presence of MB. a) Coomassie blue staining and b) anti-NFK blotting of TcAChE-containing samples. The protein (1) was mixed with MB (2) in the dark or further irradiated for (3) 1, (4) 5, (5) 10 or (6) 20 minutes. Other lanes correspond to the reaction of TcAChE plus MB in the presence of inhibitors (7) in the dark or further irradiated for (8) 1, (9) 5, (10) 10 or (11) 20 minutes.
