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. Author manuscript; available in PMC: 2013 Nov 15.
Published in final edited form as: Biochem J. 2012 Nov 15;448(1):83–91. doi: 10.1042/BJ20120992

Table 1.

Trp-containing peptides with oxidative modificationsa,b observed by LC/MSc. MS data were acquired using an MSe approach combining a low and an elevated-energy LC/MS set containing precursor ions and fragment ions, respectively.

Target Peptide sequence Modification m/z
Trp 84 72VDEQFPGFSGSEMWNPNREMSEDCLY96 +16 Da
+32 Da
+48 Da
+64 Da		 1047.33+
1052.63+
1058.13+
1063.43+
Trp 233 222AILQSGSPNCPWASVSVAEGR242 +32 Da 1109.62+
Trp 279 270KPQELIDVEWNVLPFDSIFR289 +32 Da 826.43+
268EKKPQELIDVEWNVLPFDSIFR289 +32 Da 912.03+
Trp 432/435 431VWPEWMGVIHGY442 +16 Da 745.42+
422FFNHRASNLVWPEWMGVIHGY442 +48 Da 870.13+
424NHRASNLVWPEWMGVIHGY442 +16 Da 761.43+
a

Modifications detected in MS correspond to the oxidation of several residues as follow: +16 Da = Met sulfoxide; +32 Da = NFK or two Met sulfoxides; +48 Da= Met sulfoxide plus NFK; +64 Da = two Met sulfoxides plus NFK.

b

CAM indicates derivatization to carbamidomethyl derivatives of cysteine residues detected with an additional 57 Da.

c

TcAChE was identified using the Swiss Protein Data Bank (accession code P04058).