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. 2013 Mar 8;123(4):1705–1717. doi: 10.1172/JCI64149

Figure 4. IGF-1 regulates HSPC motility in a GSK3β-dependent manner.

Figure 4

Mice were i.p. injected with PBS or 5 μg IGF-1 per mouse per day for 7 consecutive days and sacrificed 4 hours after the last injection to measure circulating WBCs (A), as well as circulating CFU cells (B) and LSK cells (C) (n = 6–9). (D) GSK3β expression (percentage of positive cells) was determined by flow cytometry in total BM cells, LK cells, and CD34– LSK cells (n = 4–8). (E) BM MNCs were obtained from PBS or IGF-1 treated mice and loaded into Transwells. Migration toward 125 ng/ml CXCL12 was assessed for 2 hours. In addition, LK cells were measured among migrating BM MNCs (n = 4–6), and CD34–LSK cells were measured among migrating Lin– cells (n = 5–7). (F) CXCR4 expression (fold change) was determined by flow cytometry in total BM cells and LK cells (n = 4).