Figure 5. IGF-1R antagonist promotes hematopoietic progenitor cell motility in a GSK3β-dependent manner.

Mice were treated with 10 mg/kg PQ401 (IGF-1R antagonist) with or without 0.6 mg/kg BIO-A or equivalent DMSO. After 1 hour, they were sacrificed and their PB was obtained to measure circulating WBCs (A), as well as circulating CFU cells (B) and LSK cells (C) (n = 4–6). (D) BM MNCs were obtained from DMSO/PQ401/PQ401 plus BIO-A–treated mice and loaded into Transwells. Migration toward 125 ng/ml CXCL12 was assessed for 2 hours. In addition, LK cells were measured among migrating BM MNCs (n = 6–8). (E) NOD/SCID mice were lethally irradiated (6 Gy) to deplete host BM CFU cells. Donor BM MNCs were obtained from DMSO/PQ401/PQ401 plus BIO-A–treated mice and then transplanted 24 hours after irradiation (5 × 10⁶ cells per mouse). After 16 hours of homing, BM was obtained and donor CFU cells were evaluated (n = 5–7).*P < 0.05 and **P < 0.01 compared with control; ^#P < 0.05 and ^##P < 0.01 compared with PQ401 treatment.