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. 2013 Mar 8;123(4):1705–1717. doi: 10.1172/JCI64149

Figure 8. GSK3β signaling regulates MT turnover in HSPCs.

Figure 8

(A) Total BM cells were pretreated with 1 μM BIO-A or equivalent DMSO for 1 hour and the content of acetylated tubulin (fold change) was determined by flow cytometry in LK cells (n = 7), and in CD34– LSK cells (n = 5). (B and C) BM MNCs or Lin– BM cells were pretreated with 1 μM BIO-A or equivalent DMSO for 1 hour, after which they were treated with nocodazole (ND, low concentration of 0.5–1 μM, or high concentration of 10 μM) or equivalent DMSO for 15 minutes and then loaded into Transwells. Migration of LK cells (B) or CD34– LSK cells (C) toward 125 ng/ml CXCL12 was assessed for 2 hours (n = 5–6). *P < 0.05 and **P < 0.01 compared with control; #P < 0.05 compared with BIO-A treatment; and ##P < 0.01 compared with CXCL12 treatment. NS, nonsignificant.