Figure 4. TCF7 mediates functions of WNT signaling in transplanted ESC-RPCs.

(A) The comparison of fTcf7 mRNA levels among ESCs, ESC-RPCs, and P-RPCs by qRT-PCR analysis. Values in ESCs were set at 1.0. (B) Protein levels of TCF7 in ESC-RPC^EGFP-derived tumors and normal adult retinae were determined by Western blot analysis. (C) The relative expression levels of neural progenitor markers and WNT signaling in ESC-RPCs, ΔNTcf7-ESC-RPCs, and P-RPCs were determined by qRT-PCR. Values in empty vector–infected ESC-RPCs were set at 1.0. (D) Effects of ΔNTcf7 on the expression of neural progenitor and proliferation markers in ESC-RPCs were analyzed. Bar charts show the quantitative analysis. Scale bar: 50 μm. (E) ERG results for eyes grafted with empty vector–infected ESC-RPCs, ΔNTcf7-ESC-RPCs, and P-RPCs. n = 10 for each group. Data are shown as mean ± SD; ANOVA (A, C, and E), t test (D); *P < 0.05, ^#P < 0.01.