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. 2013 Mar 15;123(4):1662–1676. doi: 10.1172/JCI65538

Figure 1. Liver-specific inactivation of the Glut2 gene in adult mice.

Figure 1

(A) Structure of the Glut2 floxed and CRE recombined alleles, and localization of the primers used for genotyping. (B) Western blot analysis of GLUT2 expression in the liver of mice with wild-type (WT) or floxed (G2 lox/lox) Glut2 alleles. (C) PCR analysis revealed the presence of the recombined Glut2 allele in the liver of tamoxifen-treated Glut2lox/lox;SA-CREERT2 (LG2KO) mice; no recombination was found in the kidney, brain, or duodenum of these same mice, nor in the liver of oil-treated Glut2lox/lox;SA-CREERT2 (Control) mice. (D) Western blot analysis of GLUT2 expression in liver, kidney, and pancreatic islets of control and LG2KO mice. (E) Immunofluorescence and Western blot detection of GLUT2 in the liver of control and LG2KO mice 2 and 5 months after tamoxifen treatment. A vertical line separates nonadjacent lanes from the same blot. Scale bar: 50 μm.