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. 2013 Mar 1;123(4):1542–1555. doi: 10.1172/JCI66517

Figure 7. Characterization of exosomal protein content and functional sequelae.

Figure 7

(A) Scatter plot showing exosomal and cellular protein content of MM and normal BM-MSC–derived samples. (B) MM.1S cells were exposed to exosomes isolated from MM (n = 6) or normal (n = 3) BM-MSCs for 24 hours, and IL-6 concentration was measured on conditioned media using human IL-6 ELISA. Bars indicate SD. (C) MM.1S cells were exposed to exosomes isolated from MM or normal BM-MSCs for 24 hours, and IL-6 concentration was measured in conditioned media in the presence or absence of an IL-6–blocking antibody (0.2 μg/ml) using human IL-6 ELISA. Mouse IgG2B served as isotype control. Bars indicate SD. (D) Western blot on MM (n = 6) and normal (n = 3) BM-MSC–derived exosomes using anti-fibronectin, anti–junction plakoglobin, anti-CCL2, and anti-actin antibodies. (E and F) Adhesion of MM cells to BSA (negative control), poly-d-lysine (positive control), and fibronectin-coated wells, exposed or not to MM (n = 4) or normal (n = 3) BM-MSC–derived exosomes (200 μg/ml; 6 hours). Length of adhesion was 2 hours. All data are mean ± SD of triplicate experiments. Cell-conditioned media absent cells and processed as in all samples tested served as control.