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(A–D) Whole-mount β-gal staining of CaV1.2+/LacZ embryos. CaV1.2 is highly expressed in the developing heart around E9.5 (A). By E11.5 intense staining is present in the first (arrowhead) and second (arrow) pharyngeal arches (B–D). (E and F) β-gal staining of a coronal section through the first mandibular arch. F is a magnified section of the boxed area shown in E.
