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. 2013 Mar 15;123(4):1638–1646. doi: 10.1172/JCI66903

Figure 3. Ca2+ influx through CaV1.2 regulates size of mandible in zebrafish.

Figure 3

(A) Alcian blue–stained embryos at 72 hpf. CON, uninjected control; CMO, control morpholino; MO, CaV1.2 morpholinos; MO + WT, CaV1.2 morpholinos coinjected with rabbit CaV1.2WT cRNA; MO + 4EQ, CaV1.2 morpholinos coinjected with rabbit CaV1.2 cRNA with mutations in the 4-pore glutamates (non-Ca2+ permeant); MO + TS, CaV1.2 morpholinos coinjected with rabbit CaV1.2TS cRNA. (B) Mandibular area (as outlined for CON in A normalized to the area in CMO (n = 20 for each). *P < 0.001 versus CMO. (C) Mandibular area normalized to the area in MO (n = 20 for each). *P < 0.001 versus MO. (D) Alcian blue–stained embryos at 72 hpf after treatment with DMSO or nisoldipine. (E) Mandibular area normalized to the area in DMSO (n = 20 for each). *P < 0.001 versus DMSO.