Figure 6. sox10⁺ cranial neural crest–derived cells express functional Ca_V1.2 channels.

(A) GFP images of decapitated sox10-GFP transgenic embryos at approximately 65 hpf of a control or Ca_V1.2 MO embryo. Arrowhead denotes migrating cranial neural crest. (B) Sample GFP⁺ cell after single-cell isolation. Image on the right shows a DAPI overlay. Note the GFP^– cell. (C) Whole-cell Ca²⁺ current from a GFP⁺ cell isolated from an MO or CON embryo. Voltage ramp protocol shown below. (D) Box plot showing current density for MO or CON embryos. *P < 0.01. Scale bar: 5 μm.