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. 2013 Jan 22;22(8):1264–1274. doi: 10.1089/scd.2012.0244

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 3. — Adipogenic differentiation of SSC^high-hDSPCs. Non-hDSPCs (upper panel) or SSC^high-hDSPCs (lower panel) were cultured in an adipogenic-inducing medium for 2 weeks (×400). (A) Oil-Red-O staining was positive in the differentiated SSC^high-hDSPCs. (B) Cells were stained for perilipin. Scale bar: 100 μm. (C) Oil-Red-O elution in differentiated cells. A significantly greater amount of Oil-Red-O was extracted from adipogenically differentiated SSC^high-hDSPCs than from non-hDSPCs. (D) Real-time RT-PCR analysis revealed upregulation of PPARγ, leptin, adiponectin, and FABP4 mRNA expression upon adipogenic differentiation. Values represent means±SD of independent experiments run in triplicate. *P<0.01. PPARγ, peroxisome proliferator-activated receptor-gamma; FABP4, fatty acid-binding protein 4.