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. 2013 Jan 22;22(8):1264–1274. doi: 10.1089/scd.2012.0244

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 4. — Osteogenic differentiation of SSC^high-hDSPCs. Non-hDSPCs (upper panel) or SSC^high-hDSPCs (lower panel) were cultured in an osteogenic-inducing medium for 3 weeks (×400). (A) Alkaline phosphatase staining was positive in differentiated SSC^high-hDSPCs. (B) Positive immunostaining for osteocalcin was observed in the differentiated cells. Scale bar: 100 μm. (C) Real-time RT-PCR analysis revealed upregulation of osteoglycin and osteocalcin mRNA expression upon osteogenic differentiation. The data represent the mean±SD of independent experiments run in triplicate. *P<0.01.