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. 2013 Apr;29(3):298–309. doi: 10.1089/jop.2012.0135

FIG. 4.

FIG. 4.

Aggregation assay of the FPLC- and gravity-flow purified GST-NR2E3 samples. Analysis was conducted using the Nanosep 300K Omega centrifugal device as described in the Methods section. FPLC-purified (lanes 1, 2) and gravity flow-purified (lanes 3–10) samples were diluted to the same protein concentration using the buffer containing 10 mM Tris–HCl, pH 7.5, 150 mM NaCl, 1 mM dithiothreitol, and 10% glycerol. NP-40 concentrations were adjusted as indicated in the figure. A and S lanes contain aggregated and native soluble protein fractions, respectively.