(A) The knockdown of PCBP2 inhibits the shRNA-mediated RNAi. The cells expressing either shVC or shGAPDH were transfected with siRNA against PCBP2 or siNC (control siRNA). The GAPDH mRNA levels were determined by quantitative RT-PCR. Knockdown of PCBP2 has no effect on GAPDH expression in shVC control cells. Values are mean ± SD for triplicate samples. *: p < 0.001.
(B) The knockdown of PCBP2 blocks the processing of shRNA. Values are mean ± SD for triplicate samples. *p < 0.001.
(C) The knockdown of PCBP2 blocks the processing of miRNA precursors. The panel shows the processing of miR-125a. Values are mean ± SD for triplicate samples. *p < 0.001.
See also Figure S5.