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. Author manuscript; available in PMC: 2013 Jun 6.
Published in final edited form as: Cell Metab. 2012 May 24;15(6):895–904. doi: 10.1016/j.cmet.2012.04.021

Figure 5. PCBP2 Modulates the Activity of the RNAi/MicroRNA Pathway.

Figure 5

(A) The knockdown of PCBP2 inhibits the shRNA-mediated RNAi. The cells expressing either shVC or shGAPDH were transfected with siRNA against PCBP2 or siNC (control siRNA). The GAPDH mRNA levels were determined by quantitative RT-PCR. Knockdown of PCBP2 has no effect on GAPDH expression in shVC control cells. Values are mean ± SD for triplicate samples. *: p < 0.001.

(B) The knockdown of PCBP2 blocks the processing of shRNA. Values are mean ± SD for triplicate samples. *p < 0.001.

(C) The knockdown of PCBP2 blocks the processing of miRNA precursors. The panel shows the processing of miR-125a. Values are mean ± SD for triplicate samples. *p < 0.001.

See also Figure S5.