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. Author manuscript; available in PMC: 2013 Apr 2.
Published in final edited form as: Int J Cancer. 2007 Aug 15;121(4):884–894. doi: 10.1002/ijc.22733

Figure 1.

Figure 1

Boyden chamber analysis of MDA-MB-231 and –435 human breast cancer cell lines using Matrigel or Fibronectin as a chemo-attractant. A: OS leaf extract was mixed with 100 μg /ml Fironectin or 50μg/mL Matrigel in serum free medium at concentrations ranging from 0.01–0.2% (w/v). Note the dose dependent inhibition of migration by leaf extract. B: Chemotaxis of MDA-MB-435 cells towards 50 μg /ml Matrigel in the absence or presence of 0.2–0.002% OS (w/v). C: Chemotaxis of MDA-MB-231 cells. Control: towards serum free medium; MG: towards 50μg/ml Matrigel; OS: towards Matrigel in the presence of 0.1% OS extract; LS: towards Matrigel in the presence of 0.1% Lactuca extract.

D: Migration of MDA-MB-231 cells towards Matrigel. SF: serum free medium; MG: 50μg/ml Matrigel; Eug: Eugenol; Api: Apigenin; Urs: Ursolic acid. All components and extract were mixed with MG.

E: Migration of MDA-MB-231 cells towards Matrigel: MG: 50μg/ml Matrigel; OS: 0.2% OS extract (w/v); Ap/Eu: 4.5μM apigenin and 62.5μM eugenol; Ur/Eu: 2.5 μM ursolic acid and 62.5μM eugenol; Ap/Ur: 4.5μM apigenin and 2.5μM ursolic acid; Ap/Eu/Ur: 4.5μM apigenin, 2.5μM ursolic acid and 62.5μM eugenol; K/E: 0.125 μM KOH and .001% ethanol; K/E/E: 0.125μM KOH and 0.002% ethanol. All reagents were mixed with 50μg/ml Matrigel. * P <0.001; *′ P< 0.005