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. Author manuscript; available in PMC: 2014 Jan 8.
Published in final edited form as: Cell Metab. 2013 Jan 8;17(1):101–112. doi: 10.1016/j.cmet.2012.12.006

Figure 4. AMP biosynthesis enzymes affect adenosine nucleotide derivatives.

Figure 4

(A–C) Food deprivation (Starved) and those heterozygous mutations of AMP biosynthesis components that increase lifespan also increase AMP and ADP, and reduce ATP concentration compared to controls. AMP deaminase or AICAR-ft mutations do not affect lifespan or adenosine nucleotide concentration. Transgenic overexpression of wild type AdSS (black hatched bar) rescues the alterations observed in F71 heterozygotes. Data presented ± s.e.m. (* Pπ.05, ** P<0.01, student’s t test). Throughout the figure hatched bars indicate genetic alterations that do not affect lifespan.

(D and E) Food deprivation and heterozygous mutations of AMP biosynthesis components that increase lifespan increase AMP:ATP (D) and ADP:ATP (E) ratios compared to controls. AMP deaminase or AICAR-ft mutations do not affect lifespan, AMP:ATP or ADP:ATP ratios. Transgenic overexpression of wild type AdSS (black hatched bar) rescues the increased AMP:ATP and ADP:ATP ratios observed in F71 heterozygotes. Data presented ± s.e.m. (* P<0.05, ** P<0.01, student’s t test).