(A) Flow cytometry analysis of CD25 expression on CD31+ and CD31− naïve T cell subsets demonstrated that although CD25 is expressed on a subset of CD31+ naïve T cells, there is a 1.6 fold increase in the frequency of CD25+ cells among central CD31− naïve T cell subset, four representative examples of the peripheral blood immunophenotyping from 128 donors. (B) Summary of the flow cytometry based analysis of the CD25 expression of CD31+ and CD31− naïve T cells from 26 donors (19 females and 7 males, age: 25-49). (C) Flow cytometry examination of 92 PBMCs samples (52 females, 40 males) confirmed age dependent decrease in the CD31+ naïve T cell frequency. (D-E) Flow cytometry analysis of 92 PBMCs samples, grouped in 5 year age bands, revealed age dependent acquisition of CD25 expression among both subsets of CD31+ and CD31− naïve T cells. Age effect on CD25 expression among CD31+ and CD31− naïve CD4 T cells was tested by linear regression analysis, with the phenotype, percentage of CD25+ naïve T cells, logarithm transformed to base 10.