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. Author manuscript; available in PMC: 2013 Apr 2.
Published in final edited form as: J Biol Chem. 2007 May 18;282(29):21206–21212. doi: 10.1074/jbc.M703042200

FIGURE 3. Induction of wild-type PTEN protein leads to persistence of γ-H2AX foci.

FIGURE 3

U251 cells engineered to be inducible for wild-type PTEN grown on coverslips were exposed to vehicle only (−Dox) or doxycycline for 24 h (+Dox), followed by irradiation (IR) with 4 Gy. Replicate groups of cells were fixed either immediately before IR (0) or at the indicated hours after IR. All cells were then stained for γ-H2AX or nuclei (DAPI) via immunofluorescence. Representative images of cells of each treatment group are shown in panel A. The number of γ-H2AX foci was counted for each cell and the average number of foci/cell recorded. A minimum of 300 cells in each treatment group were counted. The quantitation of the average number of γ-H2AX foci is shown in panel B. Error bars indicate S.E.