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SDS–PAGE analysis of the purification steps: (a) fractions from Ni–NTA affinity chromatography and (b) fractions from CEC. Lane M, protein molecular-weight marker (Fermentas; labelled in kDa); lane FT, flow-through; LbTXPNx, fractions corresponding to the purified protein. (c) Sequence of LbTXNPx with peptides identified by LC-MS/MS analysis shaded in grey.
