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ADSC preparation. Floating cells at 12 h (A); attached fibroblast-like cells at (B) 24 and (C) 48 h; cultured cells with large fat droplet at day 5 (D); first (E), second (G) and third (I) passages at days 7, 11 and 13, respectively; subculture cells at days 10 (F) and 12 (H). Scale bar = 200 µm.
