Fig. 3.

Immunostaining of CD marker immunoreactivity after fourth passage. As representative photomicrographs shows, CD49d is specific for ADSC (A), CD90 is a specific mesenchymal stem cells (B), CD45 is a specific for hematopoietic cells (C), CD31 for endothelial cells (D) and CD106 for BMSC (E). The left sides of the fluorescent photomicrographs represent the phase contrast images from the same field of the immunofluorescence images. The cells were immunostained with relevant primary antibodies and labeled with FITC-conjugated secondary antibody (green color shows positive cells) and the red colors are ethidium bromides for counterstaining of the nuclei. Scale bar = 200 µm.