Fig. 4.

The in vitro osteogenesis and adipogenic differentiation. (A) ADSC after incubation for 21 days in osteogenic differentiation medium. The cells were visualized with Alizarin Red S staining. The thin arrows indicate osteoblasts and thick arrows indicate the deposition of a mineralized extracellular matrix. (B) Alizarin Red S staining of ADSC before osteogenic differentiation; (C) ADSC after incubation for 21 days in adipogenic differentiation medium. The cells were visualized with Oil Red O staining. The arrows indicate adipocytes and accumulation of fat droplets; (D) Oil Red O staining of ADSC before adipogenic differentiation. Scale bar = 100 µm.