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Second derivative of the NIR spectra (7200 to 4000 cm–1) of native sugarcane bagasse (black online), pre-treated bagasse with oxalic acid (red online) and enzymatic hydrolyzed bagasse (blue online). In the OAFEX-SB, spectral region 6000 to 5920 cm–1 is assigned to the C-H stretching vibration of aromatics due to the re-localization of lignin. Changes in the polysaccharide content after OAFEX and enzyme hydrolysis at regions around 6300, 5208, 4813, 4285, and 4405 cm–1.
