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The testing of the prepared gfpIB-fetuin (A) and gfpIB-asialofetuin (B) conjugates by the evaluation of their impact on the extent of the positive hemagglutination zone. Hemagglutination-inhibition assay was designed as for optimization (Figure 1). 1 – control (sabIBs with RBC, no conjugate), 2–6 – interaction of sabIBs with conjugates and RBC; concentration of gfpIB-fetuin and gfpIB-asialofetuin conjugates is 1.5-fold increasing from 2 to 6.
