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. 2012 Nov 14;32(46):16213–16222. doi: 10.1523/JNEUROSCI.3706-12.2012

Figure 3.

Figure 3.

Genetic ablation of caspases leads to increased aneuploidy in mitotic cells from the embryonic mouse cortex. A, Analysis of metaphase chromosome counts showed increased aneuploidy in E14 caspase-3 and caspase-9-null cortices compared with wild-type cortices from littermates (100 metaphase spreads were counted per embryo; for caspase-3, n = 3; *p = 0.02; for caspase-9, n = 3; *p = 0.04, Student's t test). B, Quantitation of DNA content by flow cytometry showed a significant 3–4% decrease in overall DNA content from cortices of E14 caspase nulls compared with sex-matched, wild-type littermates (caspase-3 wild-type and nulls, n = 4, *p = 0.002, Student's t test; caspase-9 wild-type and nulls, n = 3, *p = 0.03, Student's t test). C, Histogram of the distribution of aneuploidy in E14 wild-type (black), caspase-3-null (red), and caspase-9-null (blue) NPCs. Caspase-deficient cells showed expanded distribution of numerical aneuploidies.