Table 1. Estimated number of nonsynonymous substitutions per codon (d_N), synonymous substitutions per codon (d_S), and percentage of codons under positive selection (% d_N>d_S) in peptides showing at least one positive immune reaction (epitopes) versus those showing no reactions (non-epitopes), using data from a HIV-1 subtype C high-throughput study [18].

Protein	Epitopes			Nonepitopes
	dN	dS	% dN>dS	dN	dS	% dN>dS
Gag p17	2.8±0.3	4.4±0.2*	6.5±2.4	2.8±0.4	5.4±0.3*	6.2±3.7
Gag p24	0.8±0.1**	3.9±0.3	1.4±0.9**	1.5±0.1**	4.2±0.3	5.8±1.0**
Pol RT	1.1±0.1	4.9±0.3	1.8±1.1	1.2±0.1	4.6±0.1	3.3±0.5
Pol INT	0.8±0.1	3.8±0.5	2.1±1.4	0.8±0.1	3.8±0.3	2.2±0.7
Env	2.3±1.3	4.5±1.0	11.3±3.6	3.7±0.4	5.6±0.3	10.3±1.0
Nef	1.9±0.2**	5.1±0.4	2.8±1.2*	3.1±0.2**	5.9±0.4	7.3±1.5*
Total	1.7±0.2*	4.7±0.2	4.4±0.8*	2.2±0.1*	5.0±0.1	6.2±0.4*

^*ANOVA comparing epitopes and nonepitopes: 0.01<p<0.05.

^**0.001<p<0.01.