Figure 4.
GFP fluorescence in a developing J. orithya pupal wing at the cellular level. The baculovirus vector was injected at 1.0 × 106 pfu/mL (2.0 μL) 18–24 hours post-pupation, followed by anti-gp64 antibody injection (2.0 μL) 18–24 hours post-infection. Pupal wings were dissected 24 hours post-antibody treatment. (A) A whole wing under the bright field. (B) A whole wing identical to A under blue light. Three major fluorescent clusters are indicated by arrows, two of which are shown in C and D. (C) Confocal image of a fluorescent region shown in B. A circular arrangement of the fluorescent signals is seen. (D) Confocal image of another fluorescent region shown in B, superimposed on the bright field picture.